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Ecological Systems and Devices

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OPTICAL METHODS OF THE ANALYSIS OF DIALYSIS CYANOBACTERIA FOR CONTROL OF POLLUTION OF WATER OBJECTS
I.A. Fomina, Ya.V. Savanina, E.L. Barsky, E.S. Lobakova

The possibility of non-invasive method to monitor the pollution of watercourses using dialysis culture of cyanobacteria
Synechococcus SP. PCC 6301 are examined. Biotesting method is based on measuring the difference of dichroic relations of
absorption bands of native cyanobacteria proteins cells and their external structures (index Р) recorded by infrared spectroscopy
internal refl ection. Conducted a statistical analysis of data, IR spectrophotometry showed that the selected index P
depends on the chemical composition of aquatic environment, but not the number of cells, the length of their incubation on
waterborne contaminations, volume investigated medium and type of pollutants, at least in certain intervals conditions. Use
methods of statistical analysis in the evaluation of the watercourses contamination level impact for P reduces the infl uence
of conditions heterogeneity under which carried out separate observations and get a reliable result when minimum quantity
of the studied samples. Comparison of target Δp with major assessment methods of aquatic environment pollution gives
reason to use this approach to developing advanced methods of ecological monitoring.
Keywords: cyanobacteria; dialysis cultivation; water toxicology; biotesting; IF-spectroscopy of internal refl ection.

Contacts: E-mail: irinafomina.net@mail.ru

Pp. 17-23.

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